Each compound has different characteristics, and only by selecting the characteristics of the compound suitable for a specific situation can the compound be applied on a large scale. 35202-54-1, name is 4,5-Dimethoxy-1-cyanobenzocyclobutane, This compound has unique chemical properties. The synthetic route is as follows., name: 4,5-Dimethoxy-1-cyanobenzocyclobutane
Plating on LB+agar+kanamycin, static incubation at 37° C. for 24 hours (strain 11216 of nitrilase of recombinant E. coli). (0112) Preculture in 5 ml of LB+kanamycin (50 mg/1), incubation at 37° C., 180 rpm overnight. Culture: transfer 50 ml of LB and 500 mul of preculture to non-baffled 250-ml Erlenmeyer flasks, incubation at 28° C., 160 rpm until the OD is equal to 0.6 (i.e. about 4 hours). (0113) Induction with IPTG (0.5 mM), incubation at 17° C., 160 rpm overnight (17 hours). (0114) Activity test: centrifuge the cultures at 4° C., 6000 rpm for 20 minutes, resuspend the slurry in 10 ml of 0.1M phosphate buffer pH 7. Add bicyclo[4.2.0]octa-1,3,5-triene-7-carbonitrile (10 mM)+2percent ethanol. Incubate at 220 rpm, 30° C. (0115) Note: If the culture is more than 50 ml when centrifuging, take off 50 ml and carry out the activity test using a slurry of 50 ml of culture. (0116) Hydrolysis monitoring by chiral chromatography: at 45 mins and 2 hours. Column: Phenomenex® LUNA HST 50*3 C18(2) 2.5 mum Eluant: A+B (from 0percent to 100percent B over 8 mins) A: 1000 water+25 ACN+1 TFA B: 1000 ACN+25 water+1 TFA 0.8 ml/min?40° C.?UV 210 nm Results: Monitoring by chiral chromatography shows that the reaction is not enantioselective.
Chemical properties determine the actual use. Each compound has specific chemical properties and uses. We look forward to more synthetic routes in the future to expand reaction routes of 35202-54-1.
Reference:
Patent; LES LABORATOIRES SERVIER; Pedragosa-Moreau, Sandrine; Lefoulon, Francois; (12 pag.)US9476071; (2016); B2;,
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts