Dolensky, Bohumil published the artcileMethodology of deconvolution of total solute retention on chemically modified stationary phases to structure specific contributions of bound compounds, Application of Phthalonitrile, the main research area is methodol deconvolution retention modified stationary phase structure bound compound; binding constant; bound receptor; retention factor; retention mechanism; tweezer effect; weak affinity chromatography.
The total solute retention by a chem. modified stationary phase (CMSP) has been shown several times to be a potential tool for studying the binding abilities of the bound compound In this article, we present a methodol. for the deconvolution of the total retention into structure-specific contributions. Three complementary silica-based CMSPs were prepared: (1) non-modified silica,(2) silica modified by syn-bis-Troger’s base (a mol. tweezer) and (3) silica modified by anti-bis-Troger’s base (a non-tweezer mol.). These were characterized by elemental anal. and Raman spectroscopy, and used to assemble liquid chromatog. (LC) columns. The total retention factors were estimated for electron-deficient nitro- and cyano-derivatives of benzene in both normal and reverse elution modes. The total retention factor was considered to be the sum of structure-specific retention factors, each related to the affinity (the binding constant) of a specific structure (the binding site), and its content in the modified silica, as defined for weak-affinity chromatog. (WAC). The obtained structure-specific contributions are in line with the binding studies of ligands in solution They reveal details of the retention mechanism, suggesting a more suitable attachment of ligands, and expose the shortcomings of evaluations based solely on the total retentions.
Journal of Chromatography A published new progress about Affinity chromatography. 91-15-6 belongs to class nitriles-buliding-blocks, name is Phthalonitrile, and the molecular formula is C8H4N2, Application of Phthalonitrile.
Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts