Cui, Jie published the artcileA conformational transition based fluorescent probe for mapping lysosomal viscosity fluctuations by fluorescence lifetime imaging, Computed Properties of 100-70-9, the main research area is autophagy apoptosis lysosomal viscosity fluorescence lifetime imaging.
The local viscosity within lysosomes fluctuates dynamically under various physiol. and pathol. processes (e.g., autophagy and apoptosis), and has long been regarded as a key indicator to reflect lysosomal status and functionality. Therefore, accurate monitoring of lysosomal viscosity fluctuations is essential for a better understanding of lysosomal function and pathol. Herein, we present a distorted-conformation-induced viscosity-sensitive fluorescent mol. rotor (FMR), BODIPY-P, for quant. monitoring of lysosomal viscosity. BODIPY-P exhibited outstanding performance, including high sensitivity and selectivity to viscosity, dual response mode of fluorescence intensity and fluorescence lifetime, specific lysosome targeting, quick washing-free imaging and excellent intracellular photostability. Utilizing BODIPY-P, we successfully achieved specific mapping and quantifying the fluctuations of lysosomal viscosity during autophagy and apoptosis through dual-mode imaging of fluorescence intensity and fluorescence lifetime. D. functional theory revealed that the intrinsic viscosity-sensitive mechanism of BODIPY-Pwas ascribed to the conformational transition between planar and distortion caused by the rotation of the meso-benzene ring. Our study will spur addnl. research on FMRs, including the in-depth exploration of their underlying viscosity-responsive mechanism and potential applications in viscosity-related physiol. and pathol. investigations.
Sensors and Actuators, B: Chemical published new progress about Absorption spectra. 100-70-9 belongs to class nitriles-buliding-blocks, name is Picolinonitrile, and the molecular formula is C6H4N2, Computed Properties of 100-70-9.
Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts