Tkachenko, O. V. published the artcileSynthesis and the antimicrobial activity of 1-N-alkylated derivatives of 3-N-substituted 1H-thieno[3,2-d]pyrimidine-2,4-diones, HPLC of Formula: 612-13-5, the publication is Zhurnal Organichnoi ta Farmatsevtichnoi Khimii (2013), 11(4), 15-21, database is CAplus.
Two approaches for synthesis of 3-N-substituted 1H-thieno[3,2-d]pyrimidine-2,4-diones were investigated. The first one was based on the interaction of Me 3-aminothiophene-2-carboxylate with isocyanates, which was a good way for preparation of 3-N-aryl-1H-thieno[3,2-d]pyrimidine-2,4-diones. The key step of the other one, which allowed introduction of different alkyl substituents in position 3, was oxidation of 4-oxo-2-thioxo-2,3- dihydrothieno[3,2-d]pyrimidines prepared by interaction of 3-isothiocyanatothiophene-2-carboxylate and the primary aliphatic amines with hydrogen peroxide. Alkylation of the intermediates obtained in both ways resulted in 1-N-alkyl-3-N-substituted 1H-thieno[3,2-d]pyrimidine-2,4-diones. 1H NMR spectra of the target mols. contain the signals of thiophene cycle protons H-6 (δ 8.02-8.18 ppm) and H-7 (δ 7.06-7.15 ppm) together with the signal of CH2 groups in position 1 of the heterocyclic system in the range of δ 4.70-5.20 ppm. The antimicrobial activity of the synthesized compounds was investigated by the agar well diffusion method. It was determined that the compound with Ph substituents in position 3 and o-methylbenzyl substituent in position 1 were the most active antimicrobial agent. The 1-N-alkyl derivatives of (2,4-dioxo-1,4-dihydro-2H-thieno[3,2-d]pyrimidine-3-yl)propanoic acid benzyl amide were appeared active against the strains of Staphylococcus aureus and Bacillus subtilis.
Zhurnal Organichnoi ta Farmatsevtichnoi Khimii published new progress about 612-13-5. 612-13-5 belongs to nitriles-buliding-blocks, auxiliary class Organic Pigment, name is 2-(Chloromethyl)benzonitrile, and the molecular formula is C5H7N3S3, HPLC of Formula: 612-13-5.
Referemce:
https://en.wikipedia.org/wiki/Nitrile,
Nitriles – Chemistry LibreTexts