Synthesis and Biological Evaluation of 2-(Alkoxycarbonyl)-3-Anilinobenzo[b]thiophenes and Thieno[2,3-b]pyridines as New Potent Anticancer Agents was written by Romagnoli, Romeo;Baraldi, Pier Giovanni;Kimatrai Salvador, Maria;Preti, Delia;Aghazadeh Tabrizi, Mojgan;Bassetto, Marcella;Brancale, Andrea;Hamel, Ernest;Castagliuolo, Ignazio;Bortolozzi, Roberta;Basso, Giuseppe;Viola, Giampietro. And the article was included in Journal of Medicinal Chemistry in 2013.HPLC of Formula: 64113-86-6 This article mentions the following:
Two new series of inhibitors of tubulin polymerization based on the 2-(alkoxycarbonyl)-3-(3′,4′,5′-trimethoxyanilino)benzo[b]thiophene and thieno[2,3-b]pyridine mol. skeletons were synthesized and evaluated for antiproliferative activity on a panel of cancer cell lines, inhibition of tubulin polymerization, cell cycle effects, and in vivo potency. Antiproliferative activity was strongly dependent on the position of the Me group on the benzene portion of the benzo[b]thiophene nucleus, with the greatest activity observed when the Me was located at the C-6 position. Also, in the smaller thieno[2,3-b]pyridine series, the introduction of the Me group at the C-6 position resulted in improvement of antiproliferative activity to the nanomolar level. The most active compounds I and II did not induce cell death in normal human lymphocytes, suggesting that the compounds may be selective against cancer cells. Compound I significantly inhibited in vivo the growth of a syngeneic hepatocellular carcinoma in Balb/c mice. In the experiment, the researchers used many compounds, for example, 5-Methyl-2-nitrobenzonitrile (cas: 64113-86-6HPLC of Formula: 64113-86-6).
5-Methyl-2-nitrobenzonitrile (cas: 64113-86-6) belongs to nitriles. There has been no report on the microbial biosynthesis of nitriles and the physiological function of such enzymes, nor was it not even known whether aliphatic and aromatic nitriles are biological compounds or just petrochemicals. Nitrile groups in organic compounds can undergo a variety of reactions depending on the reactants or conditions. A nitrile group can be hydrolyzed, reduced, or ejected from a molecule as a cyanide ion.HPLC of Formula: 64113-86-6
Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts