Synthesis, anticancer activity and structure-activity relationship of some anticancer agents based on cyclopenta[b]thiophene scaffold was written by Said, Mohamed;Elshihawy, Hosam. And the article was included in Pakistan Journal of Pharmaceutical Sciences in 2014.COA of Formula: C8H8N2S This article mentions the following:
Methods for the synthesis of new heterocyclic systems of thieno[3,2-d]-1,2,3-triazine derivatives and N-3-cyano-5,6-dihydro-4H-cyclopenta[b]thiophene derivatives have been developed. The newly synthesized compounds were tested in vitro against human breast carcinoma cell line (MCF-7). Compounds N-(3-cyano-5,6-dihydro-4H-cyclopenta[b]thiophen-2-yl)-2-[4-(pyrimidin-2-ylsulfamoyl)anilino]acetamide sodium salt and 4-(5,6-dihydro-7H-cyclopenta[4:5]thieno[2,3-d]-1,2,3-triazin-4-ylamino)phenol have shown the highest activity among the two synthesized series. The results of this study have led to the identification of two lead compounds with good inhibitory activities that can confirm the design of the next generation inhibitors of tyrosine kinase with fewer side effects such as hepatotoxicity and resistance. In the experiment, the researchers used many compounds, for example, 2-Amino-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carbonitrile (cas: 70291-62-2COA of Formula: C8H8N2S).
2-Amino-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carbonitrile (cas: 70291-62-2) belongs to nitriles. Trimerization of aromatic nitriles requires harsh reaction conditions, high temperatures, long reaction times, and pressure. Alkyl nitriles are sufficiently acidic to undergo deprotonation of the C-H bond adjacent to the CN group.Strong bases are required, such as lithium diisopropylamide and butyl lithium. The product is referred to as a nitrile anion. COA of Formula: C8H8N2S
Referemce:
Nitrile – Wikipedia,
Nitriles – Chemistry LibreTexts